Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.640258
Title: An investigation into the intracellular mechanisms involved in prostaglandin production by the guinea-pig uterus and placenta
Author: Aitken, Heather
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1999
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Abstract:
This thesis has studied intracellular mechanisms involved in prostaglandin (PG) synthesis by and release from the guinea-pig uterus and placenta. Adenosine, ATP and its analogous stimulated prostaglandin output (particularly PGF) from the superfused guinea-pig uterus, and from the endometrium and myometrium cultured for 24 h. Adenosine and ATP-induced PGF output from the superfused guinea-pig uterus was inhibited by the adenosine (A) receptor antagonist, 8-sulphophenyltheophylline and the P2 purinoceptor antagonist, suramin, respectively. Adenosine and ATP-induced 6-keto-PGF output from the superfused guinea-pig uterus was unaffected by 8-sulphophenyltheophylline and suramin respectively. Therefore, adenosine and ATP-induced increases in PGF output from the superfused guinea-pig uterus appear to be receptor mediated responses, while the mechanisms involved in 6-keto-PGF production remain unclear. PG output, particularly PGF2α, from the guinea-pig placenta and sub-placenta cultured for 24 h increased significantly between days 22 and 29 of pregnancy. Metabolism of PGF by the placenta also increased between these two days suggesting that increased PG output was not due to decreased metabolism. Indomethacin, a non-selective inhibitor of PGHS, and NS-398 a PGHS-2 selective inhibitor, reduced PG output from day 22 and day 29 guinea-pig placenta and sub-placenta after 24 h of culture. PGHS-2 appears to be the predominant enzyme responsible for PGF synthesis by day 22 and day 29 guinea-pig placenta and sub-placenta, while both isoforms of the PGHS enzyme are involved in PGE2 and 6-keto-PGFsynthesis by day 22 and day 29 guinea-pig placenta and sub-placenta. The outputs of PGF, and PGE2 from the placenta and sub-placenta and 6-keto-PGF output from day 29 guinea-pig placenta in culture were reduced by the inclusion of cycloheximide, puromycin and actinomycin D (protein synthesis inhibitors) in the culture medium.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.640258  DOI: Not available
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