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Title: In vitro callus selection in Brassica species
Author: Aḥmad, Shafīq
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1996
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The study mainly concerns the potential of in vitro selection as an aid to plant breeders. Three species, of the genus Brassica, were used in the investigation as model crop plants. Calli of these species were selected for NaCl tolerance. The study has three main sections dealing with the needs of tissue culture in Brassica species, in vitro selection of callus for NaCl tolerance and estimation of somaclonal variation. The first section evaluates the potential of different Brassica species and explant sources for in vitro culture. The study also describes investigations in the optimum growth regulator concentrations for continued callus cultures and subsequent plant regeneration, in these Brassica species. All the explant types i.e. leaf discs with vein, leaf discs without vein, petioles and hypocotyls, of all the species studied, showed successful callus induction and subsequent callus cultures, but with varying frequencies. Plant regeneration from callus was not successful in all the species and with all the explant sources. Curly kale (Brassica oleracea L.) showed the most successful plant regeneration. Among the explant sources, hypocotyls demonstrated the most potential with respect to plant regeneration. Optimum concentrations of NAA and BAP were different for different species. In general, BAP and NAA in balance produced more callus; and as expected, BAP enhanced shoot formation while NAA promoted root formations. The second section deals with procedures and problems associated with the in vitro selection of different Brassica species for salt tolerance.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available