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Title: Polo-like kinase interacting proteins in fission yeast
Author: Abraham, Anne
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2004
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Kms1 and Kms2 are important for integrity of the SPB. To find the biological significance of the interactions between Plo1 and these SPB proteins, attempts were made to disrupt the interaction by mutations. For this purpose, firstly regions responsible for the interaction were identified, and then mutations were made in the SPB proteins by random mutagenesis of this region. For Sid4, I isolated two point mutations, which had greatly weakened interaction with Plo1. To study the effect of disrupting the interaction in vivo, the two sid4 mutants were expressed from a fission yeast promoter in the sid4 temperature-sensitive mutant. Both point mutants of Sid4 that had weakened interaction with Plo1 were able to rescue the temperature-sensitive sid mutant with a similar strength as that of wild-type sid4 under the same promoter. The identification of potential Plo1 interacting proteins and mutants defective in these interactions will be an important step to understand cell cycle control by Plo1.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available