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Title: Investigations into the mechanisms of the fidelity of cell division
Author: Warr, T. J.
Awarding Body: University College of Swansea
Current Institution: Swansea University
Date of Award: 1991
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The principal aims of the research described in this thesis were to evaluate the application of two in vitro cytogenetic assays in detecting potential aneugenic and polyploidy-inducing agents and to further our understanding of the mechanisms of action of such agents. The cell division aberration assay employed a differential chromosome/spindle staining procedure to detect perturbations of the mitotic division apparatus. This assay was carried out in two pulmonary-derived Chinese hamster cell lines; the immortal DON:Wg3h culture and the low passage LUC2 culture. The second assay involved the quantification of metaphase chromosomes, for which only the LUC2 cell line was used, due to the stability of its diploid karyotype. Incorporated into this assay was a modified hypotonic and fixation procedure which maintained the integrity of the cell membrane and ensured against artefactual chromosome loss and gain. The two assays were used to test a battery of compounds, taken from the EEC 4th Environmental Research and Development Programme. In addition, two vinyl monomers, acrylamide and methyl vinyl sulphone were studied. The study of cell division aberrations was much less time-consuming and technically complex than the counting of chromosomes. In addition, it provided a degree of mechanistic understanding of the mode of action of some aneugenic and polyploidy-inducing chemicals. More importantly, the correlation between mitotic divisional abnormalities and the chromosome number aberrations observed at the ensuing metaphase was high for most of the compounds tested. However, the enumeration of chromosomes provides a more definitive data set for the evaluation of a chemical's aneugenic potential.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available