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Title: Thin film oxygen optical sensors and their use as biosensors
Author: Thomas, F. C.
Awarding Body: University of Wales Swansea
Current Institution: Swansea University
Date of Award: 2000
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The purpose of the thesis was to develop a sensitive oxygen optical sensor, which, when coupled with an enzymatic reaction would lead to the creation of useful optical biosensors for the biochemical analytes hydrogen peroxide and glucose. Chapter 3 is an account of work in which a [Ru(dpp)3 2+(Ph4B-)2]-based oxygen optical sensor, immobilized in silicone rubber, was developed and fully characterised. The sensor was extremely sensitive and stable towards oxygen compared with previous literature findings. The sensitivity of the oxygen sensors was temperature independent, but the response and recovery times decreased with increasing temperature. Chapter 4 gives results from an investigation into some of the factors controlling the sensitivity of a series of [Ru(L)3 2+(Ph4B-)2]-based oxygen optical sensors immobilized in cellulose acetate. The sensitivity was found to vary according to the lumophore's lifetime and the amount of plasticizer used in the formulation. The sensitivity of the oxygen sensor increased with longer lumophore lifetime and with plasticizer volume. Chapter 5 provides results from a study of an analogous, highly sensitive optical sensor, i.e. [Ru(dpp)3 2+(Ph4B-)2] immobilized in cellulose acetate butyrate and plasticized by tributyl phosphate which was coupled with an enzymatic reaction to produce a hydrogen peroxide optical biosensor. This biosensor was found to be more sensitive than those previously reported in the literature, due to the increased activity of the catalase enzyme as measured by Michaelis kinetics. Chapter 6 gives an account of the development and characterisation of a glucose biosensor. The biosensor sensitivity could be tuned for different applications by varying the amount of glucose oxidise used in the formulation. The activity, as measured by Michaelis kinetics was largely independent of temperature.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available