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Title: Soya induced gene regulation in Streptomyces lividans
Author: Thomas, D. P.
Awarding Body: University of Wales Swansea
Current Institution: Swansea University
Date of Award: 2002
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Streptomyces are soil borne bacteria. They have been shown to inhabit the soil surrounding the soybean plant. Recent studies have also indicated that Streptomyces lydicus can even form symbiotic relations with the legume Pisum sativum. It was determined that a compound originating from the soya plant is capable of upregulating transcription of the tipA gene. This represents the first evidence of molecular interaction between Streptomyces and soybean. Attempts were made to purify this compound and determine its structure. Suggestions of it nature are discussed on the basis of its chemical properties and probable mass. Possible mechanisms by which the unknown may regulate the tipA promoter were considered. The independence of this mechanism from thiostrepton-like induction was ascertained. The ability of the compound to confer a multiple antibiotic resistance phenotype was also documented. Subsequently, the role of a cytochrome P450 with respect to this phenotype was also investigated. Modulation by thiostrepton was also considered. Furthermore, an expanded version of the model for regulation of the tipA locus is discussed, including a possible role for the associated tipB gene.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available