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Title: Component analysis of natural products by Lc, Lc-Api-Ms, and Lc-Api-MSN techniques
Author: Silcocks, S. E.
Awarding Body: University of Wales Swansea
Current Institution: Swansea University
Date of Award: 2001
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Natural products provide a wide and virtually inexhaustible source of novel, useful, and often hard to synthesise compounds. However, due to the origin of these products, the matrices tend to be complex, and individual components are difficult to isolate and conclusively identify. The majority of this thesis focuses on the separation, identification, and quantification of certain species inherent within natural product samples. Liquid chromatography atmospheric pressure ionisation mass spectrometry techniques were utilised for this research. Chapter One provides a brief history and introduction to the principles and applications of liquid chromatography, mass spectrometry, and their use as an interfaced combined system. Chapter Two incorporates an investigation into the main component groups of cashew nut shell liquid (i.e. cardanol, cardol, 2-methylcardol, and anacardic acid). Compositional trends relative to the degree of applied heat treatment were examined. The compositions of certain regional samples were also compared. Chapter Three encompasses four synthesised phyto-oestrogen compounds known to naturally occur in plant-derived sources suspected as having potential cancer-protective activity. A separation of the four compounds is described, along with an account of their mass spectral identification and characterisation. Selected ion and selected reaction monitoring techniques were utilised for the determination of the limits of detection of each analyte. Chapter Four investigates the highly complex composition of propolis, a natural product manufactured by bees, and reported to provide a range of medicinal benefits. A liquid chromatographic separation of the substance is described. Additionally, differently sourced samples were mass spectrally analysed, and each one characterised and compared by their eight most abundant compounds. Chapter Five is an exception to the majority of the thesis and covers novel double-charge-transfer spectrometry investigations into the double-ionisation energies of acetonitrile and fluorinated benzene molecules. A brief appraisal of theorised values is also included.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available