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Title: A study of the influence of genetic and environmental factors upon chromosome stability
Author: Rogers, R. W. S.
Awarding Body: University of Wales Swansea
Current Institution: Swansea University
Date of Award: 2003
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The primary aim of this study was to investigate the effects of various genetic and environmental factors on chromosome stability. Several different cytogenetic techniques were applied to characterise numerical and structural chromosome changes in human cell lines under different conditions. Fluorescence in situ Hybridisation (FISH), using whole chromosome paints was used to assess chromosome changes in a set of human thyroid cancer cell lines with varying p53 status. The results highlighted the influence of p53 status and in vitro ageing upon numerical and structural instability. The second part of the study involved the application of the cytokinesis blocked micronucleus assay in combination with the immunofluorescent staining of the kinetochores and centromere specific FISH to study the genotoxic potential of Colchicine, Nocodazole, Albendazole and Taxol in AHH-1 cells. Results confirmed their anegenic potential and revealed that aneuploidy was occurring principally by chromosome non-disjunction. The final section of this research was the investigation of centrosome stability and the response on human fibroblasts with varying p53 status to UV B exposure. The p53 status of the cultures did not have any visible effect upon the number of centrosomes present in the cells. Differential staining of the spindle and the chromosomes revealed no damage to the mitotic machinery following UV B exposure. A dose and cell type dependent response in apoptosis induction was observed, this appeared to be occurring via a p53 independent mechanism.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available