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Title: Molecular population genetic studies of sturgeon species in the South Caspian sea
Author: Rezvani Gilkolaei, S.
Awarding Body: University of Wales Swansea
Current Institution: Swansea University
Date of Award: 1997
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The stock structure of three sturgeon species from the South Caspian Sea was studied. PCR-based mtDNA analysis (RFLP) was used for the study of population differentiation in the Russian sturgeon (Acipenser gueldenstaedti). The mtDNA ND5/6 gene regions was amplified using PCR techniques followed by RFLP analysis. 39 different composite haplotypes were detected among 62 specimens. 29 haplotypes were rare occurring only once in two regions (west and east areas of the Southern Caspian Sea). The average nucleotide and haplotype diversities within populations are estimated to be 0.028727 ± 0.000 and 0.9645 ± 00042 respectively and divergence between populations to be 0.00052. A highly significant difference in the distribution of haplotypes was observed between the west and east areas, suggesting the possible existence of different stocks. RAPD markers were used to discriminate different kinds of black caviar and sturgeon meat. A polymorphic band from RAPD amplification of DNA from two out of five sturgeon species was purified, cloned and sequenced. SCAR primers were designed and used to amplify caviar DNA from five sturgeon species. A marker of potential economic importance was discovered that is able to distinguish three species from their caviar and also is able to distinguish ship sturgeon caviar from osetra caviar (Russian and Persian sturgeon). This marker could assist international conservation and legal efforts to save what is left of the commercial ship sturgeon populations which are endangered and whose caviar is at present substituted for the more expensive osetra caviar. A partial sequence of the mtDNA ND5 gene region was used for population study in Persian sturgeon (west and east areas of Southern Caspian Sea). The results showed that although this approach was informative for phylogenetic study in sturgeon it was less informative for population study in Persian sturgeon.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available