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Title: The DNA repair of UV and X-ray induced lesions in selected genes of Saccharomyces cerevisiae
Author: Reed, S. H.
Awarding Body: University College of Swansea
Current Institution: Swansea University
Date of Award: 1995
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Here I examined the nucleotide excision repair of UV induced pyrimidine hydrates detected by the E. coli endonuclease III enzyme. This is not preferential in RAD cells for MAT versus HML, and is totally absent in rads1,2,3,4 and 23, which are equally defective in CPD repair. This suggests differences in the NER process depending on the type of lesion encountered. Interestingly, an allele of rad14 was shown to repair pyrimidine hydrates efficiently, while being defective in CPD repair. The rad14 deletion mutant was defective in the removal of both types of lesion. The observation that repair of pyrimidine hydrates in HML is normal in the rad7 and 16 mutants shows that the role of these proteins is not in opening the chromatin structure, since NER of certain lesions is not affected. We propose that the role of these proteins in NER is involved in the repair of GPD's or certain regions of the genome (possibly core DNA). This questions the role of redoxyendonuclease, the yeast equivalent of E. coli endo III, a DNA glycosylate capable of removing pyrimidine hydrates via the BER pathway. Strand specific repair has been reported for thymine glycol (a pyrimidine hydrate), in the induced Gal7 gene. It was presumed that this lesion was repaired via the yeast redoxyendonuclease during BER, thus extending transcription coupled repair to this pathway.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available