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Title: An investigation into the mechanisms and regulation of genetic change and DNA repair in yeast
Author: Piperakis, S.
Awarding Body: University College of Swansea
Current Institution: Swansea University
Date of Award: 1980
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The content of macromolecules (DNA, RNA and protein) was determined for temperature sensitive, radiation sensitive (to red) cultures of Saccharomyces cerevisiae. The results indicated a gradual, decrease of the macromolecular content per cell of all cultures as they progress from the log to the stationary phase. The effect of mutagens in macromolecular synthesis was investigated by growing the cultures after treatment with IN, heat and RN02. All these mutagens induce division delays which are probably due to the time required for the removal of the lesion blocking the DNA replication. The macromolecular content was however very little affected. One is rad mutant was further investigated by growing it at various temperatures and after exposure to various doses of mutagens, such as UV, heat, IINO2 and IOM as well as after "shift up" and "shift down" experiments. The results indicated that a defect which is temperature sensitive indirectly affected the repair. An attempt was made to elucidate the reason for the 18% DNA deficiency in a diploid culture of yeast in comparison to wild type. It was found that the strain has also a deficiency in its RNA and protein content. These dificiencies however could not be attributed to the sensitivity of the strain to mutagens. When the DNA density profile, after CsCl centrifugation, was established, no missing DNA fraction could be detected, suggesting that the missing DNA is probably scattered throughout the genome.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available