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Title: Kinetic models for protein adsorption on porous cylindrical particles
Author: Onugha, L. C.
Awarding Body: University of Wales Swansea
Current Institution: Swansea University
Date of Award: 2003
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Most chromatographic purification techniques for macromolecules (bipolymers) operate on the principles of adsorption. The rate of the adsorption process depends on mass transfer kinetics. Previous models of the adsorption kinetics have assumed that the adsorbent/packing particles used are spherical. Adsorbents which are fibrous or consist of rod-like particles offer potential advantages over the spherical particles. Whatman UK Ltd. market a semi-rigid cellulose range of chromatographic adsorbents for macromolecule separation (Express-Ion) which are of this type, i.e. may be likened in shape to cylinders. The major aim of this study is to develop detailed kinetic models for the pore diffusion and mass transfer processes governing the adsorption kinetics of proteins that will predict the concentration-time profiles for the adsorption in both stirred tank and packed column modes of operation using semi-rigid cylindrical adsorbents. The models have been developed to allow for particle size distribution within adsorbent samples, and for the delay and mixing that occurs in the flow line of the equipment. The stirred cell model constructed has been validated by fitting theoretical data to experimental curves obtained for the adsorption of bovine serum albumin on the anion exchanger Express-Ion D. This system was characterised by determining adsorption isotherms and the physical parameters of the adsorbent. The effects of varying the protein concentration, effective pore diffusivity, liquid film mass transfer coefficient, particle disperity, number of particle size groups, stirrer speed, and the equilibrium isotherm parameters on the adsorption profiles have been studied. A packed bed variant model has also been constructed and used to predict the effect of process parameters on the breakthrough curve in a packed bed (chromatographic) mode, and to study the effects of particle geometry on the adsorption process.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available