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Title: The applications of molecular biology to the study of environmental genotoxicity
Author: Myers, B. R.
Awarding Body: University College of Swansea
Current Institution: Swansea University
Date of Award: 1995
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The study of the environmental genotoxicity of physical and chemical agents is limited by the assays currently used to assess mutagenicity both in vitro and in vivo. For the most part these assays are phenotypic in nature and are limited to particular species and particular genes. As a result of this the data from these types of assays may not be fully representative of the ability of a particular agent to induce mutation. Furthermore, the suitability of the available in vivo mutagenicity assays for the testing of chemical agents for possible mutagenic effects at the level of DNA is not considered entirely appropriate from a regulatory point of view. The Restriction site Mutation assay (RSM assay) has been presented as a possible alternative to the phenotypic mutagenicity assays currently employed for the study of mutagenicity both in vitro and in vivo. The RSM assay is a genotypic approach to the study of the induction of restriction enzyme insensitive recognition sites by mutagens and is theoretically applicable to any species for which DNA sequence data is available. The RSM assay employs the molecular biological techniques of restriction enzyme digestion, the polymerase chain reaction and DNA sequencing to isolate and characterise these sites. The work presented in this study covers the development and testing of RSM assay protocols potentially suitable for the testing of mutagenicity of environmental chemicals in vivo in laboratory mice. A preliminary attempt at developing RSM assay protocols for use with the mussel Mytilus edulis (L.) is also reported.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available