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Title: Enhancement of fermenter design procedures based on batch culture analysis
Author: Menkel, F.
Awarding Body: University College of Swansea
Current Institution: Swansea University
Date of Award: 1994
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The objective of this work was to develop an unstructured, deterministic and uniform mathematical model of cellular activity in a liquid phase batch culture, including the initial lag phase. A new mathematical model, termed the algebraic model, has been developed for predicting cellular activity of a liquid phase batch culture. In contrast to the Monod (1949), model the algebraic unstructured mathematical rate model is based on the natural order of cellular processes, namely, substrate uptake, followed by conversion into energy and new cells. The specific substrate uptake rate is modelled by a hyperbolic Michaelis-Menten type equation, as a function of the environmental substrate concentration. Conversion of carbon substrate into new cells and energy is modelled by a variable yield concept, which incorporates the requirements of cell internal maintenance. The algebraic model can also simulate the initial lag behaviour of a batch culture. In order to test the algebraic model, a Windows based computer program has been written and was used to analyse 49 batch culture experiments of Young (1991) and 22 of Patel (1991) for the aerobic culture of Saccharomyces cerevisiae on glucose as the carbon and energy limiting substrate in a chemically defined liquid medium. These batch culture experiments were simulated by only two cellular activity phases, namely, the start phase, and the main phase. An initial linear substrate uptake phase, or start phase, was observed for all the experimental data analysed, followed by exponential substrate uptake and cell growth during the main phase. The duration of the start phase was predicted by a biomass doubling concept. The start phase ended when the biomass concentration in the batch culture had doubled with reference to the initial biomass concentration after inoculation.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available