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Title: The analysis of placental tissues for biomonitoring the population exposed to pollution from the oil fires in Kuwait
Author: Marafie, E. M. R. H.
Awarding Body: University of Wales Swansea
Current Institution: Swansea University
Date of Award: 1997
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Sophisticated molecular methods for the identification of DNA damage in response to PAHs are presented in this thesis. They were used to analyse placental samples from Kuwaiti mothers exposed to this pollution during pregnancy. 32P-postlabelling assay (PPL) has been employed to detect exposure-related DNA adducts in placental tissues from non-smoking, smoking UK mothers (negative and positive controls) and non-smoking Kuwaiti mothers exposed or unexposed to oil well fires as it represents an easily obtainable alternative tissues for human biomonitoring. It was unequivocally shown that human placental tissues from UK smoking mothers had adduct levels nearly two fold higher than levels in UK mothers who do not smoke. Analysis of placental DNA-adducts from Kuwaiti non-smoking mothers who were exposed to oil well fires showed approximately the same level of adducts in UK mothers who smoke. In order to determine whether the higher adduct levels in Kuwaiti mothers exposed to pollution during the fires was in fact due to the pollution, further analysis from Kuwaiti non-smoking mothers collected after one year of the oil well fires was undertaken. The total level of adducts in this group showed statistically no difference from that seen in individuals exposed to the pollution. Finally, the P53 mutational spectrum in placental DNA obtained from UK non-smoking, smoking and Kuwaiti non-smoking mothers exposed or unexposed to oil well fires was assessed using PCR amplification and RSM. RSM revealed seven mutations: a single transition and frameshift at codon 281-282 in exon 8 of a non-smoking mother and six transitions in codon 247-248 in exon 7 of Kuwaiti non-smoking mother exposed to oil well fires. No relationship was found between cigarette smoking and P53 mutations, and between the adduct levels and P53 mutations in Kuwaiti samples.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available