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Title: The effects of probiotic supplementation on the response of the intestinal microflora to antibiotic therapy
Author: Madden, G. F. J.
Awarding Body: University of Wales Swansea
Current Institution: Swansea University
Date of Award: 2001
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Much of this work involves the assessment of the effects of probiotic supplementation on the composition of antibiotic disrupted intestinal microfloras. In a double blind, placebo controlled clinical trial of patients undergoing Helicobacter pylori eradication therapy the effects of a probiotic comprising Lactobacillus acidophilus and Bifidobacterium longum on the faecal flora were compared. After antibiotic therapy, no change was observed among the numbers of anaerobes but the aerobic numbers increased in the antibiotic alone group; numbers of aerobes (enterobacteria) decreased in the antibiotic/probiotic group. Aerobic numbers for the probiotic re-growth population were significantly lower than the starting populations. This did not occur in the antibiotic group. In a second trial, the caecal and faecal microflora of irritable bowel syndrome (IBS) patients were compared with healthy subjects (n=8 and n=7, respectively). The composition of the caecal and faecal microfloras was comparable but numbers were lower for the caecal biopsies and caecal lumen samples. For the IBS patients, the faecal anaerobe numbers were lower than for the normal subjects and lactobacilli were not detected. The caecal samples of the IBS patients had higher numbers of aerobes than the normal subjects; lactobacilli were detected in these samples. The IBS patients were divided into two groups to receive antibiotic therapy with or without probiotic supplementation. The numbers of caecal anaerobes decreased in the antibiotic alone group but increased in the probiotic group whereas numbers of aerobes increased in both groups. Antibiotic treatment of the IBS patients favoured yeast proliferation (detected in 50% caecal samples and 75% faecal samples) but in the probiotic group, yeast growth was less abundant - only detected in 25% faecal samples.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available