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Title: Determination of the biological kinetic parameters of fermenter design from batch culture data
Author: Knights, A. J.
Awarding Body: University College of Swansea
Current Institution: Swansea University
Date of Award: 1981
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There is a need for data on the kinetics of microbial growth and substrate removal. Then the mathematical models of fermenter operation can be tested and improved. For example, the suggested incompatability of batch culture kinetic data ito continuous culture fermenter design can be reconsidered. Established methods of batch culture data analysis utilising the asymptotic and experimental solutions to the kinetics of Monod biological rate equations have been shown to be unsatisfactory. Nevertheless, an approach has been proposed by which the initial values of the kinetic parameters determined from a batch culture can be assessed in relation to the true values of Monod kinetics. Analytical time course solutions to the complete Monad biological rate equations for batch culture are available in the literature. These solutions have been obtained by making the assumption that there is a constant microbial mean yield. Gates and Mallar (1968) have proposed an analysis method for batch culture data based on these constant yield time course equations. The Gates and Warier (1968) method has been shown to give erroneous kinetic parameter values for some types of batch culture. A time course equation has been derived for the logistic microbial rate equation proposed by Edwards and Willie (1968) and Sonak (1975), and used to obtain algebraic time course solutions to the Monad biological rate equations. An analysis procedure for batch culture data has been derived from the application of simple linear regression to the logistic time course equations. The analysis method is termed the Optimization Rectangle procedure, by which a complete set of kinetic parameters can be obtained from a single batch culture experiment. The Optimization Rectangle procedure has been applied to published batch culture data for T. viride, C. tropioalis and S. oexevisiae. The kinetic parameter values obtained are equivalent to the published values for these cultures derived from complex non-linear curve fitting procedures.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available