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Title: Studies of veterinary drugs by supercritical fluid extraction, supercritical fluid chromatography and supercritical fluid chromatography-mass spectrometry in equine samples
Author: Jenkins, R. E.
Awarding Body: University of Wales Swansea
Current Institution: Swansea University
Date of Award: 1999
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Initially, the feasibility of using supercritical fluid chromatography as a means of analysing veterinary drugs was investigated. The classes of drugs that were studied included xanthins, corticosteroids and diuretics. Packed column supercritical fluid chromatography interfaced to mass spectrometry using the moving belt and thermospray interfaces was also investigated as a method of analysis. Standard mixture of the drugs were successfully separated and detected using supercritical fluid chromatography-mass spectrometry. Supercritical fluid extraction was investigated for its potential as an alternative extraction technique for horse feed. A variety of horse feeds were investigated using supercritical fluid extraction with supercritical fluid chromatography-mass spectrometry and gas chromatography-mass spectrometry as the analysis methods. Water was demonstrated to enhance extraction of caffeine and theobromine. Hordenine, caffeine and theobromine were successfully extracted from the different horse feeds. Finally, coupled supercritical fluid extraction - supercritical fluid chromatography interfaced to a mass spectrometer was investigated. It was used as a means of extracting and detecting corticosteroids from horse urine. Results obtained demonstrate the potential of coupled supercritical fluid systems in the field of drug analysis.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available