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Title: Analyses of amino acids in human urine
Author: James, M. D.
Awarding Body: University College of Swansea
Current Institution: Swansea University
Date of Award: 1995
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Analyses of amino levels in human urine were studied in order to evaluate their potential use in tests which could be used for the early detection of cancer. Three suitable reagents for derivatisation were studied and it was found that of the three reagents considered for use in this study, {dansyl chloride, dabsyl chloride and OPA}, dansyl chloride proved the most suitable as it was the most sensitive, enabled accurate reproducible separation of a greater number of amino acids than the other reagents and was the easiest of the three with which to work. A control group of 27 healthy individuals, were provided with five sample bottles in order for them to provide a number of separate samples taken throughout the day. The data that were obtained from these sample were used to establish the amino acid levels in the control group of healthy individuals. The results also demonstrate that the daily variation in amino acid levels in a healthy individual was not responsible for the difference between individuals. In the Discussion and Summary it can be seen that the pattern of concentrations of individual amino acids obtained is different for each form of cancer. Each pattern adds further evidence for the presence of a particular form of tumour. The final conclusion is that the lower average range of the amino acid alanine in cancer patients is statistically significant, it is therefore possible to use the depressed average level of alanine along with raised average level of isoleucine in data obtained from a number of samples taken from any individual patient over a period of 2-3 days in comparison with the average levels in the control group of healthy volunteers to assess a patient for the presence of any form of cancerous tumour within that individual.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available