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Title: Salt tolerance and subcellular localisation of chloride ions in Agrostis stolonifera L.
Author: Hodson, M. J.
Awarding Body: University College of Swansea
Current Institution: Swansea University
Date of Award: 1983
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The physiology of salt tolerance was investigated in two clones of the grass Aqrostis stolonifera L., one salt-tolerant clone isolated from a salt marsh, and one non-tolerant clone from an inland site. When the clones were NaCl-stressed the sodium and chloride concentrations of the shoots and roots increased, while potassium concentrations decreased. The inland clone took up more sodium and chloride into its shoot than the salt marsh clone, and at high salinity lost more shoot water. Salt grown plants of both clones had higher sodium and chloride concentrations in the older leaves than in the younger leaves and the stem. At high external (200 mM) NaCl concentrations the inland clone's older leaves dehydrated, but this was not the case in the salt marsh clone. The salt marsh clone was more tolerant than the inland clone towards all the alkali metal chlorides, magnesium chloride and calcium chloride. The effects of LiCl and KC1 on shoot and root ionic concentrations were also investigated. The effects of the interaction between salt stress and nitrogen limitation on growth and on ion, soluble nitrogen and sugar concentrations of the two clones were investigated. Several soluble nitrogenous compounds accumulated in NaCl-stressed plants (e.g. glycine betaine, proline and asparagine). Accumulation was greater in the salt marsh plants than in the inland plants. Soluble nitrogen concentrations were generally lower in plants grown with a lower nitrogen supply, but different compounds were affected to different extents. Chloride was located at the subcellular level using a silver precipitation technique. Precipitation sites in the leaves of salt-treated plants of both clones included the epidermal cells and the mesophyll intercellular spaces, cell walls, plasmodesmata and vacuoles. In the root tips precipitates were located in provacuoles, vacuoles and cell walls. The sites of precipitation were identical in the two clones.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available