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Title: Fatty acid composition and glycerolipid metabolism during temperature adaptation in the marine cyanobacterium Aphanizomenon sp.
Author: Gashlan, H.
Awarding Body: University of Wales Swansea
Current Institution: Swansea University
Date of Award: 2001
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The nature and mechanisms of adaptive changes in the fatty acid composition of membrane glycerolipids in laboratory cultures of the marine cyanobacterium Aphanizomenon sp. induced by temperature transitions similar to those experienced by natural population of Aphanizomenon in the Baltic Sea were investigated. At 28° palmitic acid (16:0) predominated (35%) over α-linolenic acid (α-18:3; 23%), whilst in cells grown at 15°, α-18:3 (38%) predominated over 16:0 (25%). Changes in fatty acid composition of the major glycerolipids monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG0, sulfoquinovosyldiacylglycerol (SL) and phosphatidylglycerol (PG) were studied after temperature shifts from 28° to 15° and from 15° to 28°. After lowering the temperature, the level of 16:0 and 18:1 decreased in MGDG, DGDG and SL fractions, whilst the content of 16:3 and α-18:3 increased. In PG however the level of 16:1 and 18:1 increased whilst the level of α-18:3 decreased. After a temperature shift from 15° to 28° the level of 16:0 remained unchanged in MGDG and SL whilst α-18:3 decreased and 16:1 increased. Determination of the positional distribution of fatty acids present in the individual glycerolipid classes using Rhizopus lipase hydrolysis established the predominance of C-18 fatty acids at the sn-1 position and C-16 fatty acids at the sn-2 position; during the adaptation to lower temperature the most prominent change at the sn-1 position was an increase in 18:1, 18:3 and 16:3 levels in MGDG.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available