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Title: The isolation and cDNA cloning of a mannan-binding lectin from the haemolymph of the cockroach Blaberus discoidalis
Author: Gallagher, S. J.
Awarding Body: University of Wales Swansea
Current Institution: Swansea University
Date of Award: 2003
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This current study describes the isolation and cDNA cloning of a lectin from the haemolymph of the cockroach Blaberus discoidalis. The lectin was isolated from serum via a mannan-Sepharose column and was found to co-purify with a number of other proteins of unknown function. Further methods to separate these proteins were investigated and the results indicated that the proteins may interact with one another forming a complex. The lectin, however, was found to have a stronger affinity towards galactose than towards mannan and so can be referred to a galactose-binding lectin. Antibody raised against this lectin was subsequently used in an immunoscreening procedure allowing the positive identification of a cDNA clone from the Blaberus cDNA library. Subsequent sequencing of the DNA demonstrated that this lectin had significant similarity to a family of lectins identified in the cockroach Periplaneta americana. Many insect lectins, such as those in Periplaneta, have been shown to function in the immune defence response, however, additional roles have been described in limb regeneration, for lectins such as regenectin. Preliminary investigations into the function of the newly isolated Blaberus lectin, failed to demonstrate a role in the opsonization and phagocytosis of bacteria and so suggest that it may have other functions.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available