Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.636998
Title: An investigation into clastogenicity at low and high toxicity
Author: Fowler, P. J.
Awarding Body: University of Wales Swansea
Current Institution: Swansea University
Date of Award: 2005
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Abstract:
The major aim of the present study was to identify groups of chemicals that were clastogenic at low and high toxicity and examine patterns of gene expression in an experimental cell line after dosing with these chemicals in order to achieve greater understanding of cellular responses to insult from these chemicals. The first part of this thesis focuses on cytogenetic identification of chemicals that are clastogenic at low and high toxicity. The micronucleus assay was chosen to compare pairs of chemicals that had the same mechanism of clastogenic action i.e. Alkylation, but different toxicity profiles. Results showed that there were two distinct groups of chemicals, those clastogenic at low and high toxicity, and those clastogenic at high toxicity only. The second part of this thesis focuses on the gene expression changes within the human cell line AHH-1 after dosing with chemicals of differing toxicity profiles. In order to examine gene expression change, cDNA arrays were used. Results showed significant change in gene expression between clastogens active at low and high toxicity. The group of chemicals clastogenic only at high toxicity had an increase in the expression of heat shock protein genes whereas the chemicals that act at low toxicity did not induce a change in heat shock protein gene expression despite dosing at up to 50% toxicity. Array results also showed a difference in DNA damage and repair signalling gene expression after insult from high and low toxicity clastogens.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.636998  DOI: Not available
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