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Title: A biochemical study of pyrazole and its derivatives in the Cucurbitaceae
Author: Flayeh, K. A. M.
Awarding Body: University College of Swansea
Current Institution: Swansea University
Date of Award: 1979
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The possible biological functions of pyrazole, B-pyrazol-l-yl-L-alanine and its peptides are discussed. It was also found that the production of the peptide of B-pyrazol-l-yl-L-alanine was dependent upon the B-pyrazol-l-yl-L-alanine content of the plant (Cucumis sativus). Studies with {3,4-14C} pyrazole indicated that pyrazole is mainly converted to B-pyrazol-l-yl-L-alanine and y-L-glutamyl-B-pyrazol-l-yl-alanine. But four other metabolites of pyrazole were also detected. The biosynthesis of the pyrazole moiety of B-pyrazol-l-yl-L-alanine has been studied in detail. Isotope dilution experiments showed that among the compounds tested 1,3-diaminopropane is the most direct precursor of the pyrazole moiety of B-pyrazol-l-yl-Lalanine. These studies also indicated that the polyamine, spermidine (w-aminopropyl-waminobutylamine) could be the precursor of 1,3-d'aminopropane, though other possible precursors were also identified. Studies with 14C-1,3-diaminopropane confirmed that this compound is converted to B-pyrazol-l-yl-L-alanine. Preliminary attempts to demonstrate the enzymic synthesis of B-pyrazol-l-yl-L-alanine from 1,3-diaminopropane have been made. This has not yet been established. Finally, a biogenetic pathway leading to pyrazole, B-pyrazol-l-yl-L-alanine and y-L-glutamyl-B-pyrazol-l-yl-alanine is suggested. 1 containing B-pyrazol-l-yl-L-alanine in Cucurbitaceae has been examined. Pyrazole could not be detected in any of these plants. The Trisodium pentacyanoaminoferrate assay used to detect pyrazole in crude plant extracts was found to be unsatisfactory because of the presence of other compounds in the plant extracts which interfere with Cucumis sativus.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available