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Title: Adhesion measurement techniques : a comparative study using latex beads and bacterial spores
Author: Fenton, Adam
Awarding Body: University of Wales Swansea
Current Institution: Swansea University
Date of Award: 2001
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The aim of the project was to develop and apply three methods of quantitatively studying particle adhesion, developed from previous related work. The Packed Column, the Spinning Disc and the Radial Flow Chamber (RFC) were chosen due to their practicality and ability to assess the effect of hydrodynamic shear on particle adhesion. The effects of variable parameters were investigated, allowing characterisation of each system. The methods were developed and used in conjunction with glass and stainless steel to assess the effect of pH on the adhesion of aminated and carboxylated latex beads, and the spores of Bacillus mycoides and Bacillus subtilis. Measurements of surface charge and hydrophobicity of the collector surface and colloidal particles were performed, and compared with the results generated using the three assessment techniques. Particle zeta potential and hydrophobicity ranged from +10mV to -40mV and 0 to 95%. Collector hydrophobicity, measured by contact angles, ranged from 21 to 76o. Surface preparation techniques provided consistent surface roughness. Particle hydrophobicity was independent of surface charge. The findings showed that adhesion agreed qualitatively using the three methods of assessment, but not quantitatively. Steel promoted adhesion more than glass, B. mycoides provided greatest adhesion followed by aminated beads, carboxylated beads, and finally B. subtilis, with the highest levels of adhesion occurred at low pH (ranging from 0 - 7000mm-2). Adhesion varied greatly (up to 70%), attributed to the heterogeneity of the particle, collector surface, and flow within each system.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available