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Title: Characterization of proteoglycans synthesized by human peritoneal mesothelial cells
Author: Yung, S. S.-Y.
Awarding Body: University College of Swansea
Current Institution: Swansea University
Date of Award: 1993
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This thesis consists of three studies on the synthesis of proteoglycans (PGs) and hyaluronan (HA) in relation to peritoneal dialysis. In the first study, newly synthesized proteoglycans from human peritoneal mesothelial cells were labelled in vitro with either 35S-sulphate, 3H-glucosamine or 35S-methionine for 24h, and were characterised using biochemical and immunological techniques. The following were identified: i). a large chondroitin sulphate proteoglycan (CSPG I), Mr > 1000 kDa, protein core > 400 kDA, which was present in both the culture medium (CM) and the cell layer extract (CL). ii). a minor dermatan sulphate proteoglycan (DSPG I), Mr 200 kDa, protein core 45 kDa, also present in the CM and identified as biglycan. iii). a second small dermatan sulphate proteoglycan (DSPG I), Mr 110 kDa, protein core 43 and 47 kDa, and found solely in theCM. This proteoglycan, identified as decorin, was thepredominant species. iv). a large heparan sulphate proteoglycan (HSPG I), Mr 1200 kDa, isolated in both the CM and in the cytoskeletal-matrix which may be related to perlecan. v). a cell surface hybrid proteoglycan (containing both HS and CS chains), Mr 190-210 kDa, and identified as syndecan. vi). a glycosylphosphatidylinositol-anchored heparan sulphate (HSPF II), Mr ≈190 kDa and identified as glypican. In addition to these proteoglycans, a large amount of free CS/HS GAGs were present (70% of the total CL). Pulse chase data revealed that these GAG chains were undergoing degradation. In the second study, proteoglycans were isolated from CAPD fluid and characterised. The results revealed that decorin was the predominant species with minor amounts of biglycan. Finally, the presence of HA in CAPD fluid was monitored. Results showed that the HA levels are elevated during peritonitis, but with successful treatment, the levels returned to normal. In vitro studies suggested that peritoneal mesothelial cells were the likely source of the HA.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available