Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.636716
Title: Analysis of degradation products of drugs and dyes by LC-MS and SFC-MS
Author: Yousef, M.
Awarding Body: University of Wales Swansea
Current Institution: Swansea University
Date of Award: 2005
Availability of Full Text:
Access from EThOS:
Abstract:
Chapter one introduces chromatography and mass spectrometry used to conduct experiments in this thesis. An explanation in the importance and ways of implementing the interfacing of high-pressure liquid chromatography and supercritical fluid chromatography with mass spectrometry is briefly summarized. The feasibility of high-pressure liquid chromatography and supercritical fluid chromatography mass spectrometry for the analysis of β-blockers is shown in chapter two. Methods were developed for the separation and analysis of various β-blockers using SFC and HPLC coupled to MS for specificity. The expected advantages of using SFC in relation to HPLC for analysis were not met, resulting in favour of the faster HPLC method. Nevertheless, SFC is complementary to HPLC as it offers advantages in rapidity of method development, reduce cost of purchasing and disposal of solvents. Chapter three demonstrates the investigation into the fading products of Azo dyes using LC-MS. The separation and analysis of standard azo dyes using LC API MS was implemented to observe the behaviour of dyes under these set conditions. The importance in characterisation of impurities at low levels is critical from a customer safety and FDA regulations aspect. Chapter four demonstrates the analysis of two unknown degradation products of methyl-5-aminolevulinate as being related to the degradation of methyl-5-aminolevulinate using high-pressure liquid chromatography mass spectrometry. Finally Chapter five describes the investigation of high-pressure liquid chromatography and supercritical fluid chromatography mass spectrometry in the characterisation of an unknown penicillin G impurity present in batch samples. The impurity in question co-eluted with the main bulk drug and could only be analysed using ion-pair agents.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.636716  DOI: Not available
Share: