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Title: Chemiluminescent probes for biological molecules
Author: Yang, J-J.
Awarding Body: University College of Swansea
Current Institution: Swansea University
Date of Award: 1994
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This work describes the synthesis of new chemiluminescent ester compounds and their applications in immunoassay. The new compounds are 2,6-dibromo-4-(2-succinimidyl-oxycarbonylethyl)phenyl 10-methylacridinium-9-carboxylate trifluoromethanesulfonate (II), 2,6-dimethyl-4-(2-succinimidyloxycarbonylethyl)phenyl 10-methylacridinium-9-carboxylate trifluoromethanesulfonate (III), 2-(2-succinimidyloxycarbonylethyl)phenyl 10-methylacridinium-9-carboxylate trifluoromethanesulfonate (IV), 4,6-dibromo-2-(2-succinimidyloxycarbonylethyl)phenyl 10-methylacridinium-9-carboxylate trifluoromethane-sulfonate (V), 4,6-dimethyl-2-(2-succinimidyloxycarbonylethyl)phenyl 10-methyl-acridinium-9-carboxylate trifluoromethanesulfonate (VI) and 4-bromo-6-methyl-2-(2-succinimidyloxycarbonylethyl)phenyl 10-methylacridinium-9-carboxylate trifluoromethane-sulfonate (VII). All these compounds contain an acridinium ester unit, which provides the chemiluminescent moiety, a phenolate unit as a leaving group and a succinimidyl ester unit as a means of linking to biological molecules. The thesis is divided into two parts. The introduction gives a broad background and understanding of chemiluminescent biological probes. Initially the process of light emission is explained in brief. This is important because it is light emission from a chemical reaction that is chemiluminescence. This is followed by a description of the major chemiluminescence reagents, especially acridinium ester species as examples, and of how these can be used as labels in chemiluminescence immunoassay. The second part describes the synthesis of compounds (II), (III),(IV), (V), (VI), and (VII), together with som basic studies of theirchemiluminescent properties and attachment to Biblogical materials.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (M.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available