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Title: Ion structure determination using novel time-of-flight techniques and mass-analysed ion kinetic energy spectrometry
Author: Williams, J. P.
Awarding Body: University of Wales Swansea
Current Institution: Swansea University
Date of Award: 2000
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The last decade has seen an important revival of one area in particular, time-of-flight mass spectrometry, which has had a significant impact on the field of mass spectrometry. This has been largely due to two recently developed ionisation methods, namely electrospray and matrix-assisted laser desorption ionisation. These ionisation methods have led to the development of novel time-of-flight mass spectrometer designs by commercial manufacturers, which take advantage of the theoretically unlimited mass range and the acquisition of a full mass spectrum every injection pulse of ions. Matrix-assisted laser desorption and electrospray ionisation have been interfaced to two novel time-of-flight mass analysers, the 'Autospec 5000', a hybrid sector orthogonal acceleration time-of-flight instrument and the 'LCT', a liquid chromatograph time-of-flight instrument, manufactured by Micromass U.K. Ltd. respectively. They have been successfully used to investigate and fully characterise complex systems of industrial significance. The 'Autospec 5000' was used for high-energy collision induced dissociation experiments. The high sensitivity of the time-of-flight analyser was very powerful in the detection of product ions produced from various synthetic polymer precursor ions. The detailed structural information produced will be shown to fully characterise the polystyrene samples studied. An expanding area of mass spectrometry is electrospray ionisation used with orthogonal acceleration, time-of-flight. The two methods when used in reflectron mode have significantly removed early limitation on resolution that time-of-flight mass analysers initially possessed. Sampling the electrosprayed ions orthogonally results in an increased duty cycle, which can be advantageous if fast chromatography is required. Evaluation of the LCT instrument will be shown to provide mass resolution of the order of 5000 at full width half maximum, mass accuracies of the order of 5 ppm, full scan sensitivity equal to that of a quadrupole instrument in single ion monitoring mode and the detection of singly charged ions greater than m/z 10,000.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available