Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.636164
Title: Analysis of polar herbicides and steroids by LC-MS using atmospheric pressure ionisation techniques
Author: Browne, E. R.
Awarding Body: University of Wales Swansea
Current Institution: Swansea University
Date of Award: 2003
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Abstract:
A brief introduction to solid phase extraction, chromatography and mass spectrometry is given. A further explanation of the ionisation processes and the interfaces used to allow for coupling of high performance liquid chromatography to mass spectrometry is provided. This thesis contains three main bodies of research involving the use of high performance liquid chromatography coupled to mass spectrometry. Firstly, the techniques were used to perform analysis of triazines (atrazine, propazine, simazine, terbutryn, trietazine), phenylureas (chlortoluron, diuron, isoproturon, linuron, methabenzthiazuron) and acidic herbicides (benazolin, bentazone, bromoxynil, dicamba, dichlorprop, ioxynil, MCPA, mecoprop, monuron). Solid phase extraction was investigated for the analysis of these herbicides in river water and analysis of real samples was conducted; detection limits in the high picogram to low nanogram per litre level were achieved. Secondly the analysis of free and conjugated estrogen steroids (β estradiol, estriol, estrone, progesterone, β estradiol 3, 17-disulfate, 17-β estradiol 3-glucuronide 17-sulfate, estrone β-D-glucuronide, estrone 3-sulfate) was undertaken. Analysis of river water extracts spiked with four conjugated estrogen steroids was performed; solid phase extraction was investigated for the analysis of free and conjugated estrogen steroids in river water and analysis of real river water samples conducted; detection limits in the high picogram to low nanogram per litre level were achieved. The third part of this thesis involves the development of a method for the determination of conjugated steroids (androsterone glucuronide, DHA 3-glucuronide, DHA 3-sulfate, β estradiol 3-sulfate, nandrolone sulfate, testosterone sulfate) in male equine urine; detection limits at the low nanogram per millilitre level were achieved. The analyses of urine samples taken before and after administration of and anabolic steroid were investigated.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.636164  DOI: Not available
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