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Title: Mitochondrial DNA length variation in the mussel Mytilus
Author: Bell, S. R.
Awarding Body: University College of Swansea
Current Institution: Swansea University
Date of Award: 1996
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Three types of mitochondrial DNA length variation in Mytilus were examined. For the purposes of this study, the three variations were termed "macro-length-variation", "micro-length-variation" and "trossulus-length variation". Macro-length-variation was observed at a frequency of 25% at the hybrid M.galloprovincialis/M. edulis population at Mevagissey on the south Cornish coast, southwest England, Mitochondrial DNA (mtDNA) was extracted and digested with a diagnostic enzyme, EcoRI. Positive results with F-genome specific PCR primers and probes indicate that the macro-length-variant is F-genome mtDNA. The normal length for Mytilus mtDNA is approx. 17.4 kb. Macro-length-variants showed mtDNA of lengths between 21.2 and 31.2 kb with an incremental increase of approx. 2.08 kb. Micro-length-variation was observed during polyacrylamide gel electrophoresis of PCR product. Sequence directed bending of the DNA helix is proposed as the cause of the electrophoretic mobility variability. Trossulus-length-variation was studied in five M. trossulus mussels taken from the Gulf of Gdansk in the Baltic Sea. PCR primers flanking the putative control region revealed incremental length increase of approx. 200 bp. PCR product from a normal-length individual and a length-increased individual was cloned and sequenced revealing a single tandem repeat of 190 bp at the extreme 3' end of the putative control region. The repeat junction aligns with a flanking tRNA (tyr) gene. RFLP analysis at a separate PCR locus revealed close similarity between the M. trossulus samples and common M. edulis haplotypes, confirming the findings of previous studies.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available