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Title: In vitro correlates of disease resistance in salmonids
Author: Marsden, Matthew J.
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 1993
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A variety of nonspecific humoral and cellular immune-parameters were investigated in several families of farmed Atlantic salmon, Salmo salar, known to exhibit differential susceptibilities to Aeromonas salmonicida (furunculosis) in the field (fishfarm) situation. Nonspecific phagocyte functions, respiratory burst activity and leucocyte migration, were not found to be selected for in resistant families. However, nonspecific humoral factors, complement, a2-macroglobulin (aa2m) and non-a2m-antiprotease activity were found to be selected for, with resistant families possessing higher activities during challenge situations. Thus, both complement and antiprotease activity (particularly a2-macroglobulin) are regarded as potential resistance markers against furunculosis. The manner in which these factors affect the disease course of furunculosis are discussed. Antigen specific cytokine assays were developed in response to A. salmonicida antigens following vaccination against this pathogen. Antigen specific proliferation and macrophage- activating factor (MAF) production were demonstrated against A. salmonicida antigens. The potential ability to screen for differential antigen specific responsiveness as a way to assess one facet of the contributions of vaccine design and selective breeding to disease resistance were investigated. Both vaccine formulation and family origin of salmonids were found to influence in the induction and magnitude of antigen specific responses. Reasons for the observed results are discussed.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available