Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.634008
Title: Investigating the causes and consequences of miR-34a dysregulation in rheumatoid arthritis
Author: Tange, Clare Elizabeth
ISNI:       0000 0004 5349 143X
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 2015
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Abstract:
Rheumatoid arthritis (RA) is a chronic, inflammatory disorder, whereby synovial inflammation ultimately results in joint destruction. Although the joints are the main target tissues affected, RA is also associated with a number of co-morbidities - such as cardiovascular disease. A key cell type involved in the perpetuation of disease pathogenesis is the macrophage, but the mechanisms underlying inflammatory gene expression in these cells are not fully understood. One fascinating and rather novel area of research that could provide insights into the activation of macrophages in RA comprises the biology of microRNA. These small, non-coding RNA molecules are implicated in the post-transcriptional regulation of gene expression. Here, we show that the expression of one particular microRNA, miR-34a, is increased in synovial fluid CD14+ cells compared to matched peripheral blood cells from RA patients. We have demonstrated that miR-34a expression is increased in synovial tissues from RA patients compared to osteoarthritis comparators, and that a proportion of these miR-34a positive cells are CD68+ macrophages. Of particular interest, miR-34a was also up-regulated in peripheral blood CD14+ cells isolated from multiple drug-resistant RA patients compared to healthy controls. Using over and under-expression methodologies we were able to demonstrate that miR-34a over-expression reduces toll like receptor-induced cytokine production by macrophages, while miR-34a inhibition enhances cytokine production. The altered cytokine activities included TNFα and IL-6 that are both critically linked to disease pathogenesis, therefore we propose that miR-34a over-expression in RA macrophages represents a failed attempt to attenuate on-going inflammation. To further explore the mechanism of miR-34a action, a microarray was performed to investigate transcripts that were regulated in response to miR-34a over-expression in monocytes. This study uncovered several pathways, including interferon, metallothionein and chemokine pathways, wherein many members were down-regulated upon miR-34a over-expression. Future work will therefore aim to dissect the role of these pathways, and their relevance to miR-34a regulated macrophage and dendritic cell biology, and thus to the chronicity of synovitis.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.634008  DOI: Not available
Keywords: Q Science (General) ; QR180 Immunology
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