Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.633366
Title: Histone modifications across the cell cycle in undifferentiated and differentiating mouse embryonic stem cells
Author: Goss, Hannah Myren
ISNI:       0000 0004 5366 0977
Awarding Body: University of Birmingham
Current Institution: University of Birmingham
Date of Award: 2014
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Abstract:
The role of post translational histone modifications in stem cells has been of increasing interest in recent years, however, the heritability of histone modifications has not yet been determined, and as such their status as epigenetic remains in question. Here we have taken the novel approach of comparing the enrichment of histone modifications, across specific genes and how they are modulated through various phases of the cell cycle: in doing so we address this question of heritability from a new perspective. Highly dynamic fluctuations in the enrichment of histone modifications were observed across the cell cycle in embryonic stem cells. In cell cycle regulated genes the patterns of modification enrichment revealed an increase in active marks either pre-emptive or at the point of expression, indicative of highly dynamic regulation, not a stable heritable transmission, perhaps reflective of the plasticity of these cells. Following on from this embryonic stem cells were differentiated for seven days, allowing the enforcement of canonical cell cycle regulation and a more lineage specific transcription profile. At this point histone modifications displayed a variety of patterns including what appeared to be the stable and presumably heritable transmission of H3K4me3 and H3K27me3 across the cell cycle.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.633366  DOI: Not available
Keywords: R Medicine (General)
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