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Title: Molecular evolution in yeast : role of chromosomal inversions and translocations in speciation, adaptation and gene expression
Author: Naseeb, Samina
Awarding Body: University of Manchester
Current Institution: University of Manchester
Date of Award: 2011
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Species belonging to the Saccharomyces ‘sensu stricto’ group present a good model for studying evolution due to their genetic versatility and availability of genomic data. Chromosomal rearrangements play a very important role in evolution of eukaryotes as well as prokaryotes as they can affect phenotypic characteristics and modes of speciation. In nature these rearrangements are most likely caused by highly mobile genetic elements, such as retrotransposons. Karyotypic changes can cause alterations in gene transcription causing genomic instabilities by inactivating or over-expressing particular genes. Only few studies looked at the importance of chromosomal rearrangements and their role on global and local gene expression. The aim of this PhD project is to investigate the impact of chromosomal inversions and translocations on fitness adaptation, gene expression and speciation. I first demonstrated that a single gene inversion (of DAL2) within a co-expressed gene cluster can cause an alteration of the expression of inverted gene as well as the neighbouring genes, ultimately leading to a phenotypic change. I also showed that small and large size pericentric and paracentric inversions do not always alter the gene expression and in general have no effect on growth rate. It was also shown that effect of large inversion on gene expression is not always localized within the inversion but occurs globally. Finally, it is demonstrated that chromosomal translocations can be responsible for the reproductive isolation of Saccharomyces paradoxus and Saccharomyces cariocanus.
Supervisor: Delneri, Daniela Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available