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Title: Novel approaches for cysteine bioconjugation
Author: Nathani, R. I.
ISNI:       0000 0004 5358 0301
Awarding Body: University College London (University of London)
Current Institution: University College London (University of London)
Date of Award: 2014
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This thesis describes and investigates novel strategies for cysteine modification to achieve protein bioconjugation. Chapter 1 provides an introduction to the research project with an overview of protein modification techniques. Chapter 2 describes the development of a site selective dual labelling strategy based on substrate controlled cysteine modification. The application of this strategy to green fluorescent protein is also detailed. Chapter 3 describes the development and evaluation of thiophosphonium as a platform for protein modification. An in-depth discussion on reaction mechanisms, pathways, stability of thiophosphonium and the utility of this platform is also included. Chapter 4 describes the development of bromomaleimide based reversible cysteine modification, with particular focus on development of improved strategies for conversion of thiomaleimides back to free cysteines. The utility of the approach was demonstrated using a proof of concept experiment.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available