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Title: Development of molecular markers linked to quantitative and qualitative assessment of bitterness in lettuce
Author: Chadwick, Martin
ISNI:       0000 0004 5346 0924
Awarding Body: University of Reading
Current Institution: University of Reading
Date of Award: 2014
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Taste is an important factor in foods, particularly minimally processed foods such as salad. The sensory profile of lettuce was assessed using members of an F9 recombinant inbred line (RIL) mapping population derived from the iceberg cultivar Lactuca sativa cv. Salinas and Lactuca serrio/a UC96US23, its wild progenitor. Customers were able to discern differences in bitterness in samples as the result of bitter sesquiterpene lactones (SLs), and sweetness from sugars, with high correlation between scores for these taste parameters and concentration of the metabolites. Customers preferred sweeter varieties over more bitter varieties. Subsequent analysis of 104 RILs and the parents of the mapping population by 'H NMR for a range of metabolites identified 285 NMR peaks representing 39 known compounds. NMR analysis was done in three environments, representing controlled environment, a restricted nitrogen field environment, and a high nitrogen field environment. The change in metabolome was assessed for each of the parents, showing variation in a range of compounds such as sugars, phenolics, fatty acids and amino acids. This was compared to other analysis methods to confirm that NMR was robust enough to quantify traits for quantitative trait loci (QTL) analysis
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available