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Title: Cytological and genetic analysis of the par2 locus of pB171
Author: Szardenings, Florian
Awarding Body: University of Newcastle Upon Tyne
Current Institution: University of Newcastle upon Tyne
Date of Award: 2012
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Prokaryotic low-copy plasmids ensure their stable inheritance through tripartite partitioning (par) loci. This study investigated the par2 locus of EPEC virulence plasmid pB171. Its ATPase ParA acts in concert with the DNA-binding partner protein ParB and the pare centromere sites to regularly distribute plasmids over the bacterial nucleoid. Dynamic oscillation of a ParA-eGFP fusion over the nucleoid was previously shown to be crucial for par2 activity. Plasmids carrying a non-functioning par2 locus are lost from the nucleoid and localise in the cytoplasm. The current model for par2 pru1itioning makes use of dynamic, filamentous structures formed by ParA, which are implicated in force generation by depolymerisation and segregation of plasmids by a pulling mechanism.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available