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Title: The regulation and function of cortical actin remodelling upon entry into mitosis
Author: Rosa, A. A. S.
ISNI:       0000 0004 5363 9262
Awarding Body: University College London (University of London)
Current Institution: University College London (University of London)
Date of Award: 2014
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Entry into mitosis is accompanied by dramatic changes in the morphology and polarity of cells, both of which entail remodelling of the cortical actomyosin cytoskeleton. Here I identified Diaphanous as the main actin nucleator required for the formation of the mitotic actin cortex in the context of a developing epithelium. I also identify the pathway involved, which we show is dependent on Pebble/RhoA and on the apical polarity regulators aPKC/Cdc42/Par6. Strikingly, following the activation of Pbl upon entry into mitosis, Cdc42 RhoGTPase and the polarity proteins aPKC and Par6 extend their domain more basally driving the cortical accumulation of Dia, while Rho directs the accumulation of cortical p-Myosin II. Taken together, these data reveal a new molecular mechanism for the concerted activation and localization of Dia via RhoGTPases and the intimate link between cortical repolarisation and the regulation of actin filament organisation.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available