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Title: In vitro-studies relating to honey as an alternative approach to wound therapy
Author: Bukhari, Mamdouh
ISNI:       0000 0004 5349 1958
Awarding Body: University of Sheffield
Current Institution: University of Sheffield
Date of Award: 2014
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A comparative study was made between Manuka honey-the main honey used as an antibacterial agent in medicine and some commercially available honeys. Among 22 types of honey, five types were found to be the most effective and these are Rainforest +30, Manuka +20, Nigella sativa, Oak and New Zealand Rewarewa. Three types of honey showed negative response to Catalase (an enzyme which breaks hydrogen peroxide), namely New Zealand Rewarewa, Manuka +20 and Rainforest +30. Exposure of honey to autoclaving generally leads to a reduction in its antibacterial activity. This suggests that hydrogen peroxide and other antibacterial factors are removed/denatured by autoclaving, but that in the case of Manuka against S. aureus and Rain forest+30 against Ps. aeruginosa, some; unknown, non-destroyed, antibacterial factor remains. The antibacterial effects of honey on Fusobacterium nucleatum (an anaerobic bacterium which is a major cause of mouth abscesses) were studied. Undiluted Manuka honey was again shown to be the most effective antibacterial honey against this pathogen. Three types of bacteria were isolated from honey as contaminants. These were identified using 16SrRNA as: Lysinibacillus fusiformis, Staphylococcus epidermidis and Sporosarcina koreensis; and were respectively isolated from Oak honey, Nigella sativa honey and Manuka honey. Some commercial products containing Manuka honey were tested for their antibacterial efficacy. With the exception of the Manuka Honey Conditioner, all of the products showed antibacterial activity against S. aureus such as Body wash, Shampoo, Manuka oil, Kanuka oil, Manuka Body Wash, bee venom with Manuka honey and Vita Complex. Similar results were obtained when Ps. aeruginosa tested. However, in all cases, the antibacterial effect on both bacteria was more pronounced against S. aureus than it was against Ps. aeruginosa. The antibacterial effect of the three mouth-cleaning products was then tested against some bacterial mouth flora. The Manuka and Aquafresh tooth paste showed inhibition zones for all three bacteria, while the non-honey mouthwash had no effect on bacterial growth. Four types of honey were diluted from 80% to 5% to determine the minimum biofilm eradication concentration (MBEC). At a concentration of 20% all honeys killed both types of biofilm bacteria. Some pathogenic traits such as pyocyanin production and biofilm formation were found to be influenced by a range of honeys. Various wound dressings and wound treatments were tested for their antibacterial activity against planktonic and biofilm of S. aureus and Ps. aeruginosa. Panaderm, a mixture of antibiotics, was the most effective treatment against S. aureus and Ps. aeruginosa. Activon Manuka exhibited marked activity against S. aureus and moderate activity against Ps. aeruginosa. Flamazine showed moderate action against S. aureus and Ps. aeruginosa. The proprietary (non-honey based) hand gel showed weak action on both types of bacteria.
Supervisor: Wainwright, Milton Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available