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Title: DNA barcoding and population genetic structure of Malaysian marine fishes
Author: Jaafar, Tun Nurul Aimi Mat
ISNI:       0000 0004 5365 5211
Awarding Body: Prifysgol Bangor University
Current Institution: Bangor University
Date of Award: 2014
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The development of a widely available global database of DNA barcodes has been proposed as a species-identification tool for large taxonomic assemblages of animals. The approach has particular value in revealing cryptic species, which typically have high incidence in marine environments. Despite the wealth of DNA barcode data for fish from many temperate regions, there are relatively few such data available for SE Asian waters. In Chapter 2, an initial reference DNA barcode library was produced for the marine fish Family Carangidae, one of the most commercially-important families from the Indo-Malay Archipelago (IMA). Thirty-species of Family Carangidae were collected from the IMA to examine the accuracy of DNA barcoding concepts and protocols, such as ease of amplification of the barcode gene cytochrome c oxidase I (CO/), and implementation of the 'barcoding gap' concept for species delimitation. All described species formed monophyletic clusters in Neighbour-joining phylogenetic tree, although three species representing complexes of six potential cryptic species were detected. Within 723 individuals, three described species (Atule mate, Selar crumenophthalmus and Seriolina nigrofasciata) exhibited conspecific divergences up to ten times greater (4.32-4.82%) than mean estimates (0.24-0.39%) indicating a discrepancy with assigned morphological taxonomic identification, and the existence of cryptic species within the IMA. Additional conspecifics sequences available from other geographical regions revealed the existence of several more complexes of potentially cryptic species outside the IMA. However, to explain the hypothesis of species richness in the IMA, it is necessary to sample the whole family across their broad geographic range. Such information will contribute to the development of an integrated taxonomic framework, thus informing management strategies for subsequent conservation and management of Carangidae. Additionally, the results will provide greater understanding of recruitment, and processes driving species diversification in the IMA.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available