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Title: Functional genomic analysis of the periodic transcriptome in the developing Drosophila wing
Author: Liang, Liang
Awarding Body: Open University
Current Institution: Open University
Date of Award: 2013
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The eukaryotic cell cycle is the central molecular oscillator underlying tissue growth. Although previous studies have analyzed global cell cycle-associated transcription using unicellular systems, precisely how cell cycle-dependent processes are integrated in multicellular development in vivo remains unclear. One multicellular context-specific cell cycle event is the Interkinetic Nuclear Migration (IKNM), a conserved process by which proliferating nuclei translocate to the apical epithelial surface ofthe epithelium to execute mitosis. How cell cycle progression is linked with IKNM remains poorly understood. Here, I rePort the global cell cycle-associated transcriptomes of Drosophila wing disc epithelial cells (multicellular system) and cultured S2 cells (unicellular system). With an integrative F ACS-microarray technique, we identified over 600 genes with periodic express ion profiles in each context. Intriguingly, despite the common periodic genes identified, we also identified 200 genes 'periodically expressed only in the wing disc cells, including many core cell cycle components. I further explored the function of those wing disc periodic genes by tissue-specific RNAi knockdown. Combining flow cytometry and confocal imaging, I defined 107 periodic genes that control wing growth, wherein 35 periodic genes control cell cycle progression in the developing wing but not in S2 cells (compared with results from S2 RNAi screens).
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available