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Title: Salinity tolerance and transcriptomics in rice
Author: Hossain, Mohammad Rashed
ISNI:       0000 0004 5362 7923
Awarding Body: University of Birmingham
Current Institution: University of Birmingham
Date of Award: 2014
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Morpho-physiological characterization and whole genome transcript profiling of rice genotypes that belongs to sub-species Indica, Japonica and wild relatives were carried out under salt stress. The existence of qualitatively different mechanisms of salt tolerance across the genotypes was identified. Multivariate analysis was applied to categorize the genotypes according to their level of tolerance. Modified SAM analysis elucidated the trait specific expression of genome wide transcripts. Gene ontology enrichment analysis identified the genes involved in different molecular functions such as signal transduction, transcription factor and ion homeostasis etc. Gene network analysis identified the regulatory network of genes that are active in different tissues. The differential expression of transcripts of four tolerant and two susceptible Indica genotypes under stress were further analysed. The candidate genes for different biological processes and molecular functions are identified and discussed. Highly induced stimulus responsive gene Os01g0159600 (OsLEA1a) and Os05g0382200 (Nhx) can be mentioned for instance. The differentially expressed genes that are located within the salt stress related QTLs were also identified. The transcriptomics data were also used to predict the salinity tolerance of genotypes using OSC-PLSDA model. The combined physiological and transcriptomic approach of this study gives a complementary whole organism assessment of plants responses to salt stress.
Supervisor: Not available Sponsor: Commonwealth Scholarship Commission
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QH426 Genetics ; S Agriculture (General)