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Title: Infrared multiple photon dissociation spectroscopy on biomolecular ions
Author: Boer, Tjalling Sybren Joannes Antonius de
Awarding Body: University of Oxford
Current Institution: University of Oxford
Date of Award: 2013
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In this thesis the application of Infra Red Multiple Photon Dissociation spectroscopy to the 3- dimensional structural analysis of gas-phase neutral and protonated biomolecules is described. Tuneable, infra-red lasers were used to interrogate the biomolecules in the Amide A, 1, and Il spectral regions which contain information regarding inter- and intramolecular interactions, and hence are indicative of the molecular structure. A home-built laser desorption time-of-flight mass spectrometer was used to create and analyse the neutral species of interest. ensuring minimal fragmentation. Protonated species were generated using a photochemical protonation technique, developed in the Oxford research group, after which the resulting ions were analysed in the time-of-flight mass spectrometer. Experiments in the Amide I and JI spectral regions were performed using a Free Electron Laser, which was coupled to an FTICR mass spectrometer. The analysis and structural interpretation of the data involved the comparison of the experimental spectra with those obtained from high-level quantum chemical calculations
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available