Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.606172
Title: The investigation and application of ice recrystallization inhibitors as cryoprotectants
Author: Deller, Robert C.
Awarding Body: University of Warwick
Current Institution: University of Warwick
Date of Award: 2013
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Abstract:
There is a continuing need for improvements in the cryopreservation of clinically relevant cells, tissues and organs as advances in transplantation science and regenerative medicine rise alongside an aging populace that intensifies demand. Antifreeze (glyco)proteins (AF(G)Ps) and antifreeze proteins (AFPs) are classes of proteins found in cold acclimatized species. Ice recrystallization is a highly damaging process that occurs upon the thawing of frozen specimens with AF(G)Ps and AFPs limiting this effect in a process termed ice recrystallization inhibition (IRI). However AF(G)Ps and AFPs largely fail to improve in vitro and ex vivo cryopreservation due to their secondary property of dynamic ice shaping. The biocompatible and synthetically accessible polymer poly(vinyl alcohol) (PVA) has been shown to process a strong IRI activity. The IRI property of PVA along with numerous other polymers and polyols is investigated to highlight the uniqueness of PVA (Chapter 2). PVA is then explored as a cryoprotectant with red blood cells (Chapter 3), immortalized mammalian cell lines (Chapter 4) and primary cells (Chapter 5) with a significant advantageous effect observed with each cell type in terms of the number of cells recovered post thaw. However, this is despite the use of proportionately low concentrations of PVA compared to traditional membrane permeable cryoprotectants. The application of PVA as a cryoadjuvant could therefore improve the cryopreservation of cells, tissues and organs resulting in widespread clinical benefits.
Supervisor: Not available Sponsor: Engineering and Physical Sciences Research Council (EPSRC) ; University of Warwick
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.606172  DOI: Not available
Keywords: R Medicine (General) ; TP Chemical technology
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