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Title: A role for microRNA-155 in the control of infection
Author: John, V. F.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2011
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MicroRNAs (miRNAs) function through targeted binding to the 3’ un-translated region (UTR) of messenger RNAs (mRNAs) in a sequence specific manner. Recent studies have implicated microRNA-155 (miR-155) as an important player in the development and function of a number of immune cells including B and T cells, macrophages and dendritic cells. The aim of this study was to investigate the role of miR-155 in controlling either a mucosal Citrobacter rodentium or a systemic Salmonella enterica serovar Typhimurium infection in the context of the overall immune response. Here we present evidence that miR-155-deficient mice are less competent in their ability to eradicate a mucosal C. rodentium infection compared with wild type control C57BL/6 mice. We show that miR-155-deficient mice have a higher C. rodentium burden in gastrointestinal tissue and also exhibit spread into systemic tissues. Additionally, we demonstrate that germinal centre formation and humoral immune responses are impaired in the absence of miR-155. In view of the fact that this phenotype is largely reproduced in μMT (B cell-deficient) mice reconstituted with miR-155-deficient B cells we conclude that miR-155 is required to control C. rodentium infection. Further, miR-155-deficient mice were able to clear a primary infection with an attenuated strain of S. Typhimurium but were defective in their immune response to Salmonella.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available