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Title: The integrated microfluidic cell and the magnetically encoded planar tags
Author: Jiang, Z.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2007
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This dissertation reports the design, fabrication and testing of a novel bio-sequencing device termed by the author as the Integrated Microfluidic Cell (IMC), and the invention of the novel SU-8 encapsulated Magnetically Encoded Planar Tags (MEPTs). The IMC is designed to be a platform where multiplex bio-assays take place and particles are detected and sorted magnetically. It features MR sensors incorporated into the channel, so as to read out single bit or multibit information carried by magnetic particles in flow. The magnetic particles first reach a focusing gate where they are aligned along the centre of the channel and are guided over the sensors one by one. After that, they are sorted into different sub-branches of the channel by a stripline-based sorting gate. Results are presented for two generations of the IMC. The essential difference between the two generations is, for the first generation IMC, i.e. the AMR based IMC, AMR ring and block sensors were used; for the second generation IMC, i.e. the GMR based IMC, PSV ring sensors were used. Besides that, there are also many other improvements in the GMR generation. A separate bead sorting experiment was done to test the efficiency of the sorting gate with a mixture of magnetic and non-magnetic beads, and the sorting gate worked well with an error rate of only 8%. Signals were not obtained from the AMR based IMC, which might be due to insufficient sensitivity of the AMR sensors. For both GMR based IMCs, the PSV ring sensors were accidentally damaged by static charge, and were not rebuilt in time due to the complexity of the device. However, the principle of such a bead sorting device and the bio-sequencing techniques are well proved.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available