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Title: The design and analysis of magnetic tags for high throughput biological analysis
Author: Hong, B.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2011
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A design of a novel biological assay platform is presented in this thesis for the application of high throughput DNA sequencing and multiplexing biological assays. Digital magnetic carriers are responsible for the labelling and carrying of biological molecules or DNA strands through the microfluidic cells for various chemical reaction and analysis. This carrier design contains a bit addressable “magnetic barcode” made of patterned magnetic thin film (e.g. Permalloy or Cobalt), sandwiched in between two rigid SU8 polymer protective layers. I utilized magnetic elements with strong shape anisotropy and different coercive fields as the data storage elements, so each one of the magnetic barcodes elements can be addressed individually by a single globally applied variable magnetic field. The main idea being that all the carriers can be made using only a single photolithography template, which is particularly suitable for low cost mass production. The “label” or “signatures” can be coded or re-coded to the carriers in real time as and when needed. This approach is ideal for labelling the oligonucleotides during the split-and-mix synthesis or for the combinatorial library-based high-throughput multiplexed bioassays. I describe how the design of the magnetic barcode is optimized by engineering the coercivity of each barcodes element, allowing the number of available signatures to be increased. I also show that by using a globally applied magnetic field and the magneto-optical Kerr microscope, the magnetic elements in the multi-bit magnetic carriers can be addressed individually and encoded/decoded remotely. The power of this approach is the read/write technique, which allows modest globally applied magnetic fields to write almost unlimited numbers of codes to a large population of carriers rather than individuals.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available