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Title: Distribution and function of somatostatin receptors in the rat central nervous system
Author: Holloway, S.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 1997
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Following the localisation of messenger RNA (mRNA) and sst2 receptor protein distribution within the CNS, this study sought to identify functional effects of SRIF within the rat CNS and to characterise the receptor subtypes involved. Following the pharmacological characterisation of the novel sst2 receptor selective radioligand, [125I]-BIM-23027, in binding studies on rat hippocampal and striatal membranes, the autoradiographical distribution of the sst2 receptor in the rat CNS was studied and found to be widespread, particularly in limbic regions. [125I]-BIM-23027 binding sites were also found in the dorsal horn of the spinal cord, consistent with a previously suggested role for SRIF in antinociception. The distribution of [125I]-BIM-23027 binding sites in the CNS closely matched that seen using an sst2 receptor-selective polyclonal antibody (Schindler et al, 1996b), and verified the use of [125I]-BIM-23027 as a selective radioligand for the study of sst2 receptor distribution within the rat CNS. In summary, this study has shown: (1) a specific distribution for sst1-sst4 receptor mRNA in the CNS, (2) the suitability of the novel radioligand [125I]-BIM-23027 for the study of the sst2 receptor both in radioligand and autoradiographical studies, (3) that SRIF does not act presynaptically to regulate NA release from the rat hippocampus and (4) that SRIF can inhibit spontaneously firing MHb neurones via activation of sst2 receptors. The widespread presence of SRIF receptor mRNA and receptor protein in the rat CNS, in addition to the inhibitory effect of SRIF in the MHb is supportive of a role for SRIF in central neurotransmission.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available