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Title: Magnetic cell labelling and manipulation in two and three dimensional cell cultures
Author: Ho, V. H. B.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2011
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A simple and non-specific labelling technique was developed. Cell membrane proteins were first biotinylated and then bound to streptavidin paramagnetic particles. The results of characterisation studies showed that the degree of cell labelling could be precisely controlled by varying the amounts of added paramagnetic particles and the labelling is effective, quick and independent of cell uptake. The cell surface bound particles were subsequently internalised and studies showed that this labelling method did not have any drastic effect on cell viability. The labelled cells were measured to have significant magnetic moments in an applied field. They could be targeted and positioned precisely with magnetic field gradients. Highly defined cell patterns were produced using magnetically labelled HeLa, TE671 cells and human monocytes. Spatially segregated HeLa and TE671 cells were also successfully co-cultured on the same plate using this technique. Multi-layering of labelled cells could also be achieved to construct multicellular structures. The labelled cells were further cultured to generate viable magnetic multicellular spheroids. These spheroids could be easily and quickly separated magnetically without the need for centrifugation. They could also be patterned using magnetic fields in a few seconds and the patterned spheroids then fused together to form a larger tissue construct. Magnetic spheroids were also used as tumour models for drug delivery studies. These cell labelling and magnetic spheroid culture methodologies can be adapted to a wide variety of mammalian cell types. The labelled cells can then be manipulated in two dimensional cell culture or as three dimensional magnetic multicellular spheroids.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available